Academic Research Journal of Biological Sciences and Medicinal Plants
ARJBSMP
Academic Research Journal of Biological Sciences and Medicinal Plants (ARJBSMP)
Vol. 13(1), pp. 1 - 5, June, 2025.
DOI: 10.14662/ARJBSMP2025.030
ISSN:
Research Paper
Assessment of Anti-inflammatory Potential Activity of Aqueous-Methanolic Extract of Annona muricata (soursop) Leaves on Liver Function Parameters in Formalin Induced Pain Model
Md. Akande, Titilayo1*, Salawu, Sule. Ola2, Ahemen, Rejoice Seember1
11Department of Biochemistry, College of Biological Sciences, Joseph Sarwuan Tarka University, Makurdi, Nigeria. 2Department of Biochemistry, School of Life Sciences, Federal University of Technology, Akure, Nigeria. * to whom correspondence should be addressed: titilayomi2013@gmail.com or akande.titilayo@uam.edu.ng; +2348020790765 or +2347038636049
Accepted 15 June 2025
| Abstract |
The The present study was aimed to assess the anti-inflammatory activity of aqueous-methanolic extract of soursop (Annona muricata) leaves on liver function parameters in formalin-induced inflammation and oedema in male wistar rats. Inflammation and oxidative stress contribute significantly to liver damage. Soursop (Annona muricata) leaves have been traditionally used for their medicinal properties. Thirty male rats were divided into six groups: normal saline, formalin-induced, and two treatment groups receiving 100 and 300 mg/kg of soursop leaf extract while the other two groups receives Diclofenac and Aspirin (standard drugs) respectively. Liver function parameters Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Alkaline Phosphatase (ALP) and Total protein were assessed. The result of the liver function test indicated that formalin induction into the right hind paw of the rats led to significant increase at (p<0.001) in serum levels of Aspartate aminotransferase (AST) 103.87 ± 1.66f), Alanine aminotransferase (ALT) (98.57 ± 0.88e, f), and Alkaline phosphatase (ALP) (139.70 ± 0.91f) in formalin –induced not treated rats compared to the normal control and treated group rats. These results therefore implied that formalin adversely affected liver function enzymes. Treatment with aqueous - methanolic extract of soursop (Annona muricata) leaves plant extract at the doses of 100 mg/kg and 300 mg/kg body weight respectively ameliorated the ALT (93.50 ± 1.44e at 100 mg/kg; 74.70 ± 2.66b at 300 mg/kg) and AST (81.13 ± 1.13e at 100 mg/kg; 71.63 ± 0.96b at 300 mg/kg) levels associated with formalin-induced hepatotoxicity, with the effect being more pronounced at the higher dose of 300 mg/kg. Also, there was a highly significant reduction in serum AST (75.53 ± 0.23c) and ALT (82.87 ± 2.17c) concentrations (p<0.001) in diclofenac sodium treated rats when compared to the formalin-induced not treated rats. The highly significant dramastic reduction of ALP (68.90 ± 2.89b) seen at 300 mg/kg of aqueous - methanolic extract of soursop (Annona muricata) leaves plant extract treated rats showed that the plant extract has the potential to resuscitate the hepatocytes from damage, A relatively lower ALP level (85.30 ± 2.14c) was also recorded for diclofenac sodium. This study demonstrated the anti-inflammatory activity of soursop leaf extract in protecting against formalin-induced liver damage, suggesting its potential as a therapeutic agent.
KEYWORDS: Annona muricata, anti-inflammatory, AST, ALT, ALP, formalin, inflammation.
Cite This Article As: Akande, T., Salawu, S. O. and Ahemen, R. S. (2025). Assessment of Anti-inflammatory Potential Activity of Aqueous-Methanolic Extract of Annona muricata (soursop) Leaves on Liver Function Parameters in Formalin Induced Pain Model. Acad. Res. J. Bio. Sci. Med. Plants. 13(1): 1-5.